Circ_0003645 serves as miR-335-5p sponge to promote the biological process of diffuse large B-cell lymphoma by upregulating NFIB

Circ_0003645 serves as miR-335-5p sponge to promote the biological process of diffuse large B-cell lymphoma by upregulating NFIB

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Background Circular RNAs (circRNAs) are critical regulators for the lolasalinas.com development of many tumours, including diffuse large B-cell lymphoma (DLBCL).However, the role and mechanism of circ_0003645 in DLBCL progression remains obscure.Methods Quantitative real-time PCR was performed to measure the expression of circ_0003645, microRNA (miR)-335-5p and nuclear factor I/B (NFIB).Cell viability, apoptosis and cell cycle were measured by cell counting kit 8 assay and flow cytometry.

Protein expression was assessed using western blot analysis, and cell glycolysis was evaluated by detecting glucose consumption and ATP/ADP ratios.Besides, dual-luciferase reporter assay and RIP assay were used to confirm RNA interaction.Results Our data showed that circ_0003645 expression was significantly upregulated in DLBCL tumour tissues.After circ_0003645 knockdown, the viability, cell cycle and glycolysis of DLBCL cells were inhibited, while cell apoptosis was promoted.

MiR-335-5p could be sponged read more by circ_0003645, and NFIB was confirmed to be a downstream target of miR-335-5p.Function analysis revealed that anti-miR-335-5p reversed the regulation of si-circ_0003645 on DLBCL cell progression, and NFIB overexpression also abolished miR-335-5p-mediated the biological functions of DLBCL cells.Conclusion The present study revealed that circ_0003645 promoted the proliferation and glycolysis of DLBCL cells by the miR-335-5p/NFIB axis, which might provide a novel insight for DLBCL treatment.